Determination of eight components in Lamiophlomis rotate by UPLC method and preliminary study on influencing factors of contents.
10.19540/j.cnki.cjcmm.2017.0216
- Author:
Yan-Mei GOU
1
;
Shi-Hong ZHONG
1
;
Rui GU
2
;
Can ZHAO
2
;
Chun-Sheng YANG
2
Author Information
1. Pharmaceutical School, Chengdu Medical College, Chengdu 610500, China.
2. Ethnic Medical School, Chengdu University of Traditional Chinese Medicine, Chengdu 611131, China.
- Publication Type:Journal Article
- Keywords:
Lamiophlomis rotate;
UPLC;
contents determination;
iridoid glycosides;
phenethyl alcohol glycoside
- From:
China Journal of Chinese Materia Medica
2017;42(24):4807-4813
- CountryChina
- Language:Chinese
-
Abstract:
To establish a method for simultaneous determination of the contents of sesamoside, menthyl chloride, chlorogenic acid, caffeic acid, 8-O-acetyl-phloridzin methyl ester, forsythiaside B, luteolin, and ergosterol, the LIPLC analysis was performed on a Kinetex XB-C₁₈ (4.6 mm×50 mm,2.6 μm)column with acetonitrile (A)-0.1% phosphoric acid solution (B) as the mobile phase. The flow rate is 1.0 mL min⁻¹. The detection wavelength was 238, 320 and 350 nm and column temperature was 30 ℃. The results showed that the eight components could be completely separated with good linear relationships (r≥0.999 8) in the linear range. The average recoveries were 96.61%-100.4%. The method was simple with strong specificity and high precision. It can be used for the identification and quality evaluation of the Lamiophlomis rotate.The results also showed that there was a correlation between the various components, and the habitat was the main factor affecting the content of the eight components. However, the degradation gradient had no obvious effect on the contents, while the content of sesamoside was significantly decreased after one year of storage.
