Origin of neointimal cells in autologous vein graft in rat model.
- Author:
Yong-Zhi DENG
1
;
Su-Jian LIU
;
Li MA
;
Hong-Fang LI
;
Yi-Fan LI
;
Zong-Quan SUN
;
Jia-Jun CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Anastomosis, Surgical; Animals; Carotid Artery, Common; surgery; Hyperplasia; Jugular Veins; pathology; transplantation; Male; Models, Animal; Rats; Rats, Sprague-Dawley; Transplantation, Autologous; Tunica Intima; pathology
- From: Chinese Journal of Surgery 2007;45(20):1424-1427
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the potential cell sources of neointimal cells in autologous vein graft in rat model.
METHODSVein graft neointimal cell origins were investigated using a model of vein-to-artery interposition modal. Slides were stained with hematoxylin and eosin, immunohistochemical staining was also performed with primary antibodies alpha-smooth actin or CD34.
RESULTSNeointimal thickening was greater at the proximal ends (65.2 +/- 4.6) microm and, to a lesser extent, distal ends (64.7 +/- 5.3) microm, in comparison to the middle of the graft (63.5 +/- 5.6) microm. Vein-originating cells survived and make a contribution to neointimal formation within the vein graft, mostly adjacent to the lumen, suggesting an intimate association with endothelial cells, donor arterial smooth muscle cells or circulating progenitor cells.
CONCLUSIONSVein graft neointimal cells arise predominantly from vein-derived endothelial cells, donor arteria smooth muscle cells or circulating progenitor cells. It suggests clinical relevance of stenosis-inhibiting therapies directed at the vein graft or early system pharmacologic administration.
