Changes of amino acids neurotransmitters in striatum of hemi-parkinsonian rhesus monkey after high frequency stimulation of subthalamic nucleus.
- Author:
Xu-Dong ZHAO
1
;
Xiao-Ping ZHOU
;
Han-Hua LIU
;
Bao-Ming LI
;
Xiao-Wu HU
;
Feng-Qian LI
;
Ben-Ming YOU
Author Information
- Publication Type:Journal Article
- MeSH: 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine; Amino Acids; metabolism; Animals; Chromatography, High Pressure Liquid; Corpus Striatum; metabolism; Deep Brain Stimulation; methods; Disease Models, Animal; Glutamic Acid; metabolism; Macaca mulatta; Microdialysis; Neurotoxins; toxicity; Neurotransmitter Agents; metabolism; Parkinson Disease, Secondary; chemically induced; metabolism; therapy; Subthalamic Nucleus; Taurine; metabolism; gamma-Aminobutyric Acid; metabolism
- From: Chinese Journal of Surgery 2007;45(24):1682-1684
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the influence of high frequency stimulation of the subthalamic nucleus on the levels of amino acids neurotransmitters in striatum of hemi-parkinsonian monkeys.
METHODSTwo rhesus monkeys were successfully prepared for the subsequent microdialysis sessions. Collecting the dialysate before turning on the pulse generator, and collecting at 1 week, 1, 8 and 12 months after high frequency stimulation of the subthalamic nucleus. The level of Glu, GABA and Tau were determined by high performance liquid chromatography and fluorometric detection (HPLC-FD).
RESULTSAfter high frequency stimulation (HFS), PD symptoms of monkeys significantly improved. The levels of Glu in putamen and caudate nucleus of electrodes side at 1 week, 1, 8 and 12 months were increased significantly. The levels of GABA in putamen and caudate nucleus of electrodes side at 1 week, 1 month increased significantly compared with before turning on the pulse generator while decreased at 8, 12 months. The level of Tau in putamen and caudate nucleus increased significantly.
CONCLUSIONLong-term STN HFS can increase the level of glutamate and taurine, while decrease the level of GABA in putamen and caudate nucleus of electrodes side. It improves symptoms of hemi-parkinsonian rhesus monkey significantly.
