Effect of qingxin kaiqiao formula and saponin on learning and memory abilities and expression of apoptosis signal transducers Abeta and betaAPP in AD rat brain.
- Author:
Sheng-hui CHU
1
;
Hai-yan HU
;
Zhan-wang TAN
;
Xiang CHEN
;
Wen-hua WANG
;
Xiao-yan ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Alzheimer Disease; drug therapy; genetics; Amyloid beta-Peptides; genetics; Animals; Apoptosis; drug effects; Caspase 3; metabolism; Cerebral Cortex; drug effects; metabolism; Disease Models, Animal; Drugs, Chinese Herbal; administration & dosage; pharmacology; Hippocampus; drug effects; metabolism; Learning; drug effects; Male; Maze Learning; drug effects; Memory; drug effects; Rats; Rats, Sprague-Dawley; Saponins; administration & dosage; pharmacology; Time Factors
- From: China Journal of Chinese Materia Medica 2012;37(19):2947-2950
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of qingxin kaiqiao formula and saponin on the learning and memory ability and the expression of the apoptosis signal transducers Abeta and betaAPP in AD rat brain.
METHODThe comparative observation method was adopted for the animal test. Forty male SD rats were randomly divided into five groups, namely the normal group, the model group, the aricept group, the qingxin kaiqiao formula group and the saponin group, with eight rats in each group. Abeta(25-35) (10 g x L(-1)) was injected into their bilateral amygdala to establish the AD rat model. Since the next day, they were intragastrically administered with Aricept (1.67 mg x kg(-1)), Qingxin Kaiqiao decoction (12.67 mL x kg(-1)), saponin (6.30 mg x kg(-1)) and double distilled water filling for 2 weeks to observe their spatial memory ability in a Morris water maze and study the expression of Caspase-3, Abeta and betaAPP in brain tissues by immunohistochemistry.
RESULTEach traditional Chinese medicine groups showed significant improvement in the learning and memory ability of AD rats and notable differences (P < 0.05, P < 0.01) compared with the control group. The qingxin kaiqiao formula group and the saponin group showed a decrease in the expressions of Caspase-3, Abeta and betaAPP in cerebral cortex and hippocampus area, displaying notable differences (P < 0.01, P < 0.05) compared with the control group.
CONCLUSIONqingxin kaiqiao formula and saponin can obviously improve the learning and memory ability of AD rats with by decreasing the expression of Caspase-3, Abeta and betaAPP in cortex and hippocampus.
