Effect of 8-methoxypsoralen on PHA-induced DNA synthesis of human lymphocyte in the absence of UVA.
- Author:
Jin Ho CHUNG
;
Kyu Han KIM
;
Jai Il YOUN
;
Joo Heung REE
- Publication Type:Original Article
- MeSH:
DNA*;
Humans*;
Lymphocytes*;
Methoxsalen*
- From:Korean Journal of Dermatology
1992;30(5):601-607
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
We have observed the dark effect of 8-methoxypsoralen (8-MOP) on the viability and DNA synthesis in human lymphocyte cultures after stimulation with phytohemagglutinin (PHA) in the absence of ultraviolet A radiation. The concentrations of 8-MOP was 0.5-3.2 microgram/ml. We have also measured the LDH activity in supernatants of lymphocyte cultures treated with 8-MOP. The results were as follows: 1. There was no 8-MOP dose-dependent decrease in the viability of lymphocytes up to 8MOP 32microgram/ml. 2. There was 8-MOP dose-dependent decrease in PHA-induced DNA synthesis of lymphocytes from the concentration of 8-MOP 2microgram/ml. 3. There was a time-dependent decrease in PHA-induced DNA synthesis of lymphocytes at the conscentration of 8-MOP 32microgram/ml. 4. There was no LDH release in supernatant of lymphocyte cultures after incubation with 8-MOP up to 8-MOP 32microgram/ml.
