Inflammatory skin response to photochemotherapy: ear swelling response in C57BL mouse model.
- Author:
Joo Heung REE
;
Jai Il YOUN
- Publication Type:Original Article
- Keywords:
8-methoxypsoralen;
DNA
- MeSH:
Animals;
DNA;
Ear*;
Humans;
Lymphocytes;
Methoxsalen;
Mice;
Mice, Inbred C57BL*;
Photochemotherapy*;
Skin*
- From:Korean Journal of Dermatology
1992;30(5):608-615
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
We have observed the dark effect of 8-methoxypsoralen(8-M(P) on the viability and DNA synthesis in human lymphocyte cultures after stimulaticn with phytohemagglutinin (PHA) in the absence of ultraviolet A radiation. The concentratioiis of 8-MOP was 0.5-32 ug/ml. We have also measured the LDH activity in supernatant. of lymphocyte cultures treated with 8-MOP. The results were as follows: 1. There was no 8-MOP dose-dependent decrease in the viability of lymphocytes up to 8- MOP 32pg/ml. 2. There was a 8-MOP dose-dependent decrease in PHA-induced DNA synthesis of lymphocytes from the concentration of 8-MOP 2 ug/ml. 3. There was a time-dependent decrease in PHA-induced DNA synthesis of lymphocytes at the concentration of 8-MOP 32 ug/ml. 4. There was no LDH release in supernatant of lymphocyte ciltu es after incubation with 8-MOP up to 8-MOP 32ug/ml.
