Effect of Ligusticum wallichii-containing serum on expressions of Toll-like receptor 4 and myeloid differentiation factor 88 in hepatic stellate cells.
- Author:
Hai-lan WANG
;
Juan HE
;
Wen-fu CAO
;
Wen-long CHEN
- Publication Type:Journal Article
- MeSH:
Animals;
Female;
Hepatic Stellate Cells;
drug effects;
metabolism;
Ligusticum;
Lipopolysaccharides;
pharmacology;
Liver Cirrhosis, Experimental;
drug therapy;
Myeloid Differentiation Factor 88;
genetics;
physiology;
Phytotherapy;
RNA, Messenger;
analysis;
Rats;
Rats, Sprague-Dawley;
Toll-Like Receptor 4;
genetics;
physiology
- From:
China Journal of Chinese Materia Medica
2015;40(11):2191-2194
- CountryChina
- Language:Chinese
-
Abstract:
To observe the effect of Ligusticum wallichii-containing serum on the expressions of Toll-like receptor 4 and myeloid differentiation factor 88 in hepatic stellate cells. Clean-grade SD rats were randomly divided into 5 groups and orally given L. wallichii decoction, colchicine and normal saline for 7 d to prepare L. wallichii-containing serums. Except for the blank group, all of the remaining groups were stimulated with LPS 1 mg x L(-1) for 24 h. After being intervened, the L. wallichii-containing serums were cultured in 5% CO2 incubator at 37 degrees C for 24 hours. The expression of TLR4 and MyD88 were detected by RT-PCR and Western blot. After HSC was stimulated with LPS, TLR4 and MyD88 mRNA and protein expressions were significantly higher than the blank control group (P < 0.01). After being intervened with L. wallichii-containing serum, TLR4 and MyD88 mRNA and protein expressions were notably lower than the model group (P < 0.05 or P < 0.01). In conclusion, L. wallichii-containing serum could regulate the TLR4 signaling pathway and show the anti-fibrosis effect by inhibiting the expression of TLR4 and MyD88 in LPS-induced HSCs.
