HRM identification of Chinese medicinal materials Mutong.
- Author:
Jun HU
;
Zhi-lai ZHAN
;
Yuan YUAN
;
Lu-qi HUANG
;
Yong LIU
- Publication Type:Journal Article
- MeSH:
Chemistry Techniques, Analytical;
methods;
DNA, Plant;
chemistry;
genetics;
Genotype;
Magnoliopsida;
chemistry;
classification;
genetics;
Phylogeny;
Transition Temperature
- From:
China Journal of Chinese Materia Medica
2015;40(12):2304-2308
- CountryChina
- Language:Chinese
-
Abstract:
High resolution melting (HRM), an important technology for genotyping and mutation scanning, has broad prospects in the authentification of traditional Chinese medicine. This paper selected universal trnH-psbA primers and used HRM to establish a new methods for identification of Akebia herbs. PCR was conduct at the annealing temperature of 58 degrees C and 35 cycles. The range of the DNA template concentration, the primer concentration and the Mg2+ ion concentration were further analyzed. The results showed the Tm values of Caulis Akebiae was (81.84 ± 0.16), (85.28 ± 0.16) degrees C and Caulis Clematidis Armandii was (83.22 ± 0.19) degrees C and Caulis Aristolochiae manshuriensis was (81.67 ± 0.14) degrees C, (84.24 ± 0.10) degrees C with 5-125 mg - L-' DNA template, 0.4 μmol x L(-1) primer, 2.0 mmol x L(-1) Mg2+. This method can achieve the authentification of Akebia herbs and is simple, fast, high-throughput, visual.
