Chemical constituents from stems of Hedyotis hedyotidea and their immunosuppressive activity.
- Author:
Tian-tian ZHANG
;
Sha-sha GAO
;
Jun-jie HOU
;
Yong-qin ZHOU
;
Jie-wen ZHOU
;
Xiao-gang WANG
;
Nan QIN
;
Jia-chun CHEN
;
Hong-quan DUAN
;
Jin-bo FANG
- Publication Type:Journal Article
- MeSH:
Animals;
Drugs, Chinese Herbal;
chemistry;
isolation & purification;
pharmacology;
Hedyotis;
chemistry;
Immunosuppressive Agents;
chemistry;
isolation & purification;
pharmacology;
Lymphocytes;
drug effects;
immunology;
Male;
Mass Spectrometry;
Mice;
Mice, Inbred C57BL;
Molecular Structure;
Plant Stems;
chemistry
- From:
China Journal of Chinese Materia Medica
2015;40(12):2357-2362
- CountryChina
- Language:Chinese
-
Abstract:
Hedyotis hedyotidea has been traditionally used for the treatment of arthritis, cold, cough, gastro-enteritis, headstroke, etc. But few studies have screened the active compounds from extracts of H. hedyotidea. In this study, the structure of the chemical constituents from stems of H. hedyotidea were determined and the immunosuppressive activity of the compounds was evaluated. The compounds were separated and purified with silica gel, gel column chromatographies and preparative HPLC, and their structures were identified by spectral methods such as MS and NMR. Eleven compounds were obtained and identified as(6S,9S) -vomifoliol (1), betulonic acid (2), betulinic acid (3), betulin(4), 3-epi-betulinic acid (5), ursolic acid (6), β-sitosterol (7), stigmast-4-en-3-one (8), 7β-hydroxysitosterol (9), (3β,7β) -7-methoxystigmast-5-en-3-ol (10) and morindacin (11). This is the first report of compounds 1, 2, 4, 8, 9, 10 and 11 from H. hedyotidea. Compounds 1, 2 and 8-11 were firstly isolated from the genus Hedyotis, and compounds 9 and 10 were isolated from the family Rubiaceae for the first time. The immunosuppressive activity of these compounds was tested using the lymphocyte transsormationtest. Compounds 4, 6 and 9 showed significant immunosuppressive activity.
