Effect of aralosides to contraction function and calcium transient of ischemia/reperfusion myocardial cells.
- Author:
Miao-di ZHANG
;
Gui-bo SUN
;
Hui-bo XU
;
Min WANG
;
Xiao-bo SUN
- Publication Type:Journal Article
- MeSH:
Animals;
Aralia;
chemistry;
Biological Transport;
drug effects;
Calcium;
metabolism;
Drugs, Chinese Herbal;
administration & dosage;
Humans;
Male;
Muscle Contraction;
drug effects;
Myocardial Ischemia;
drug therapy;
metabolism;
physiopathology;
surgery;
Myocardial Reperfusion;
Myocytes, Cardiac;
drug effects;
physiology;
Rats;
Rats, Sprague-Dawley;
Saponins;
administration & dosage
- From:
China Journal of Chinese Materia Medica
2015;40(12):2403-2407
- CountryChina
- Language:Chinese
-
Abstract:
To discuss the protective effect of aralosides (AS) on I/R-induced rat myocardial injury. The adult rat ventricular myocyte ischemia model was established through perfusion with sodium lactate perfusate and reperfusion with Ca(2+) -containing Tyrode's solution simulation. The cell contraction and ion concentration synchronization determination system was applied to detect the effect of AS on single I/R cell contraction and Ca2+ transients. According to the findings, AS could increase resting sarcomere length, contraction amplitude, ± dL/dt(max), calcium transient amplitude and speed of post-reperfusion myocardial cells (P < 0.05, P < 0.01), and decrease in time for achieving 90.0% of maximum relaxation, time for achieving peak value, resting calcium ratio, contraction period [Ca2+] i, time for achieving 50.0% of maximum relaxation and attenuation rate of intracellular calcium transient (P < 0.05, P < 0.01). Therefore, it is suggested that AS improved the post-reperfusion cell contraction and injury of calcium homeostasis.
