Study of screening pseudoallergenic substances of Shuanghuanglian injection.
- Author:
Yan YI
;
Yu-shi ZHANG
;
Chun-ying LI
;
Hai-yu ZHAO
;
Hong-bin XIAO
;
Gui-qin LI
;
Yu-ting LU
;
Jia-yin HAN
;
Yong ZHAO
;
Hong-jie WANG
;
Nan SI
;
Ai-hua LIANG
;
Bao-lin BIAN
- Publication Type:Journal Article
- MeSH:
Animals;
Drug Hypersensitivity;
etiology;
Drugs, Chinese Herbal;
adverse effects;
analysis;
Furans;
adverse effects;
Glucosides;
adverse effects;
Glycosides;
adverse effects;
Injections;
Male;
Mice;
Mice, Inbred ICR
- From:
China Journal of Chinese Materia Medica
2015;40(14):2727-2731
- CountryChina
- Language:Chinese
-
Abstract:
In this study, chemistry, biology and pharmacology were combinated to screen pseudoallergenic substances of Shuang-huanglian injection (SHLI) so that to establish a scientific and systematic approach to screen pseudoallergenic substances of traditional Chinese medicine injections. The mouse pseudoallergic reaction models were used to screen the pseudoallergic reaction of SHLI's intermediate extract and the intermediate extract's component or ingredient. Among the three intermediates of Shuanghuanglian injection (extract of Scutellaria baicalensis, extract of Lonicera japonica, extract of Forsythia suspensa) , pseudoallergic action of Forsythia suspensa was the strongest, Forsythia suspesnsa's pseudoallergic reaction mainly associated with the composition with largerchemical polarity. Further it was found that forsythiaside A and arctiin which existed in the the composition with largerchemical polarity caused obvious pseudoallergic reactions. SHLI with removal forsythoside A with the technology of HPLC-MS displayed reduced pseudoallergic reaction and a significant improved safety. This study provided a scientific basis for SHLI process improvements and also offered idea and research foundation for screening pseudoallergenic substances injections in other TCM injections.
