Cardiotoxicity study of Shenfu compatibility in rats based on metabonomics.
- Author:
Jia-le HE
;
Jia-wei ZHAO
;
Zeng-chun MA
;
Qian-de LIANG
;
Yu-guang WANG
;
Hong-ling TAN
;
Cheng-rong XIAO
;
Tang XIANG-LIN
;
Yue GAO
- Publication Type:Journal Article
- MeSH:
Animals;
Biomarkers;
Cardiotoxicity;
Drugs, Chinese Herbal;
toxicity;
Glutathione;
blood;
Least-Squares Analysis;
Male;
Metabolomics;
methods;
Principal Component Analysis;
Rats;
Rats, Wistar
- From:
China Journal of Chinese Materia Medica
2015;40(14):2743-2747
- CountryChina
- Language:Chinese
-
Abstract:
To research the effect of Ginseng Radix et Rhizoma and Aconiti Lateralis Radix Praeparata compatibility on cardiac toxicity in rats by UPLC-Q-TOF/MS, and explore the endogenous markers and molecule mechanism. Different compatibility of Shenfu decoction were given to male Wistar rats at dosage of 20 g · kg(-1) for 7 days, collected the serum, and analyze the endogenous metabolites effected by Shenfu formulation by principal component analysis and partial least-squares analysis. Results showed that content of glutathione, phosphatidylcholine and citric acid decreased in mixed-decoction group, while ascorbic acid, uric acid, D-galactose, tryptophan, L-phenylalanine increased. The results showed cardiac toxicity of Aconiti Lateralis Radix Praeparata in Shenfu mixed-decoction. Shenfu co-decoction group showed a similar or weaker trend compared with control group, but most of them do not have a statistically significant. The results indicated the scientific basis of Shenfu compatibility by comparison of co-decoction group with mixed-decoction group. Shenfu compatibility can reduce cardiac toxicity induced by Aconiti Lateralis Radix Praeparata, and citric acid, glutathione, phosphatidyl choline, uric acid might be regarded as potential markers of cardiotoxicity.
