Studies on digestive enzyme activity of Whitmania pigra in different months old.
- Author:
Hong-zhuan SHI
;
Hong LIU
;
Qiao-sheng GUO
;
Jia WANG
;
Fei LIU
;
Meng-meng LI
- Publication Type:Journal Article
- MeSH:
Age Factors;
Amylases;
metabolism;
Animals;
Leeches;
enzymology;
Lipase;
metabolism;
Peptide Hydrolases;
metabolism
- From:
China Journal of Chinese Materia Medica
2015;40(14):2796-2799
- CountryChina
- Language:Chinese
-
Abstract:
Studies on the variation of amylase, lipase and lrotease activity of Whitmania pigra in 0-6 months old using 3, 5-dinitro- salicylic acid colorimetry, right-nitrophenyl palmitate ester (ρ-NPP) colorimetry and folin-phenol method. The results showed that pro- tease activity remained low before 1.5 months old and with the highest activity in 2 months old, but after showing a small peak in 4 months, alkaline protease rapid declined. Amylase was low at born, then gradually increased the activity of the highest in 2.5 months old. Lipase with a strong vitality at birth, then 1 month with minimum and 2 months peaked, but appeared a small peak in 4 months old. In summary, only lipase exhibits strong activity at birth, lipase with the strongest activity in the digestive tract during develop- ment. Protease, lipase and amylase with the strongest activity at 2-3 months old, but were decreased after 4 months old.