Overexpression of β(1)-adrenoceptor can not protect rat cardiomyocytes from injury induced by isoprenaline.
- Author:
Ying WANG
1
;
Feng ZHOU
;
Chuan-Ying XU
;
Hong SUN
Author Information
1. Department of Pathophysiology, Xuzhou Medical College, Xuzhou 221002, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Cell Survival;
Cells, Cultured;
Female;
Heart Failure;
metabolism;
Isoproterenol;
pharmacology;
Male;
Myocardial Contraction;
drug effects;
Myocytes, Cardiac;
cytology;
metabolism;
pathology;
Rats;
Rats, Sprague-Dawley;
Receptors, Adrenergic, beta-1;
genetics;
metabolism;
Transfection
- From:
Acta Physiologica Sinica
2010;62(6):505-510
- CountryChina
- Language:Chinese
-
Abstract:
The purpose of this study was to investigate the effect of the overexpression of β(1)-adrenoceptor (β(1)-AR) on the contractile function and cell survival of rat cardiomyocytes injured by isoprenaline (ISO). The rat cardiomyocytes were isolated using the collagenase perfusion method and then transfected with β(1)-AR gene using adenoviruses vector. Four hours after the infection, the rat cardiomyocytes were treated with ISO for 24 h to imitate the high catecholamine levels of chronic heart failure. Western blot was performed to measure the protein expression of β(1)-AR. The percentages of rod cells were measured to test cell survival. Video-based edge-detection system was used to measure the contractile function of the cardiomyocytes. The results indicated that the expression of β(1)-AR in β(1)-AR-transfected cardiomyocytes was significantly increased compared with that in control group (P<0.01). Meanwhile, β(1)-AR transfection also increased β(1)-AR protein levels in ISO-injured cardiomyocytes. The cardiomyocyte survival was significantly decreased in ISO group compared with that in control group. β(1)-AR-transfection alone had no effect on cardiomyocyte survival in β(1)-AR group, but it further decreased cardiomyocyte survival in β(1)-AR+ISO group. Contractile amplitudes of ISO-injured cardiomyocytes were significantly decreased regardless of whether they were transfected with β(1)-AR or not, although β(1)-AR-transfected cardiomyocytes showed significantly increased contractile function compared with control group (P<0.05). These results suggest that the overexpression of β(1)-AR has no significant protective effect on rat cardiomyocytes injured by ISO.
