Proliferation and IFN-gamma secretion of autologous T lymphocytes stimulated by myeloid leukemia cells induced with rhGM-CSF and rhIL-4.
- Author:
Yan-Hui XIE
1
;
Qin-Fen CHEN
;
Yi XIE
;
Hong XIE
Author Information
1. Department of Hematology, Huashan Hospital, Fudan University, Shanghai 200040, China.
- Publication Type:Journal Article
- MeSH:
Adult;
Aged;
Aged, 80 and over;
Female;
Granulocyte-Macrophage Colony-Stimulating Factor;
pharmacology;
Humans;
Interferon-gamma;
biosynthesis;
Interleukin-4;
pharmacology;
Leukemia, Myeloid;
immunology;
Lymphocyte Activation;
drug effects;
Male;
Middle Aged;
Recombinant Proteins;
pharmacology;
T-Lymphocytes;
drug effects;
immunology
- From:
Journal of Experimental Hematology
2002;10(6):496-498
- CountryChina
- Language:Chinese
-
Abstract:
To observe the proliferation of T lymphocytes stimulated by CML and AML cells which were induced by rhGM-CSF and rhIL-4, and the secretion of IFN-gamma from proliferated T lymphocytes, the expression of CD80, CD86 and HLA-DR on CML and AML cells induced by GM-CSF and IL-4 was assayed by flow cytometry in vitro. Then one-way mixed lymphocyte reaction was carried out, with induced leukemia cells as stimulating cells and auto-T lymphocytes as reactive cells. The secretion of IFN-gamma from T lymphocytes was determined by double antibody sandwich ELISA. The results showed that GM-CSF and IL-4 significantly upregulated the expression of CD80, CD86 and HLA-DR on CML cells and CD80 and CD86 on AML cells, which could stimulate the T lymphocyte proliferation and high secretion of IFN-gamma (in CML group) of autologous T lymphocytes. It is concluded that the CML and AML cells induced by GM-CSF and IL-4 have the ability to present tumor specific antigen to auto-T lymphocyte.
