Effect of p53 gene on telomerase activity and hTERT gene expression in HL-60 cells.
- Author:
Nai-Nong LI
1
;
Yuan-Zhong CHEN
;
Min LIANG
;
Lian-Huang LÜ
Author Information
1. Fujian Institute of Hematology, Union Hospital Affiliated to Fujian Medical University, Fuzhou 350001, China.
- Publication Type:Journal Article
- MeSH:
Apoptosis;
DNA-Binding Proteins;
Gene Expression;
Genes, p53;
physiology;
HL-60 Cells;
Humans;
RNA, Messenger;
analysis;
Telomerase;
genetics;
metabolism
- From:
Journal of Experimental Hematology
2002;10(6):527-530
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the change of telomerase activity and human telomerase reverse transcriptase (hTERT) gene expression in HL-60 cells transfected with wild type p53 gene, wild type p53 gene was introduced into HL-60 cells by Lipofectin transfection. Apoptosis was analyzed by TUNEL assay. Telomerase activity and the level of hTERT mRNA were detected by telomeric repeat amplification protocol (TRAP)-ELISA and RT-PCR, respectively. The results showed that the apoptotic rate of HL-60-pN53cG cells was 8.3% and 21.0% respectively after cultured at 32.5 degrees C for 24 h and 72 h. The level of hTERT mRNA was decreased to 68.4% and 55.8% and telomerase activity to 27.3% and 8.9% of control value in HL-60-pN53cG cells at the same points. In conclusions, hTERT mRNA and telomerase activity were down-regulated in HL-60 cells transfected with p53 gene. This may be one of mechanisms of apoptosis induced by wild type p53 gene.
