Significance of serological test of blood group in nonmyeloablative allogeneic peripheral blood stem cell transplantation of patients with acute leukemia.
- Author:
Hai-Lin WANG
1
;
Bao-Fu SHI
;
Min XU
;
Xiao-Hong YANG
;
Wei LIU
;
Guo-Zhong REN
;
Yan LU
;
Guo-Yan XU
;
Nai-Wu LÜ
;
Yan-Jun CAO
Author Information
1. Department of Blood Transfusion, The General Hospital of Shenyang Military Area, Shenyang, 110016, China.
- Publication Type:Journal Article
- MeSH:
Adult;
Blood Group Antigens;
Blood Transfusion;
Female;
Humans;
Leukemia, Myeloid, Acute;
blood;
therapy;
Middle Aged;
Peripheral Blood Stem Cell Transplantation;
Precursor Cell Lymphoblastic Leukemia-Lymphoma;
blood;
therapy;
Transplantation, Homologous
- From:
Journal of Experimental Hematology
2002;10(6):557-560
- CountryChina
- Language:Chinese
-
Abstract:
The purpose of this study is to explore the clinical significance of RBC blood group serological test in nonmyeloablative allogeneic peripheral blood stem cell transplantation (NAPBSCT) of ABO group incompatibility in 4 patients with acute leukemia. ABO and MN blood group of donors and recipients were determined by hemagglutination test and Rh blood group by Diana Gel phenotype Rh card. The changes of blood group in recipients were observed and implant of donor cells was monitored by short tandem repeat-PCR method. The results showed that in 2 cases of 4 recipients the marrow cells appeared mixed chimera of donor and recipient cells, and blood group changed to donor type in 1 of the 2 cases on 100 days after transplantation. In another 2 cases, the marrow cells appeared mixed chimera without blood group chimera on 154 days after transplantation, and rejection of the transplant occurred in 1 of the 2 cases. The determination of hemagglutinin titer showed that the implant rate of donor cells was lower in the recipients with higher hemagglutinin titer and blood group chimera did not appear, conversely, the implant rate was higher in the recipients with lower titer and blood group chimera appeared early. It is concluded that examination of RBC blood group in NAPB SCT can indirectly reflect effectiveness of transplantation, contribute to decide the intensity of conditioning protocol and immunosuppressive therapy after transplantation, estimate prognosis and guide blood transfusion during transplantation.
