The effect of separating red blood cells from bone marrow graft in vitro by methylcellulose.
- Author:
Miao ZHANG
1
;
Bo-Long ZHANG
;
Hai-Jie JIN
;
Chun-Ji GAO
;
Fang-Ding LOU
;
Wan-Ming DA
Author Information
1. Department of Hematology, The General Hospital of PLA, Beijing 100853, China. miao-zhang@hotmail.com
- Publication Type:Journal Article
- MeSH:
Bone Marrow Transplantation;
methods;
Cell Separation;
methods;
Erythrocytes;
immunology;
Humans;
Methylcellulose;
pharmacology
- From:
Journal of Experimental Hematology
2002;10(6):561-563
- CountryChina
- Language:Chinese
-
Abstract:
It has need to separate red blood cells (RBC) from marrow graft in ABO group unmatched BMT and auto-BMT with purging tumor cells, the separating effect of methylcellulose was observed. The mixture of 0.5% methylcellulose and bone marrow was laid up in an open transfusion system, and then sedimentation of RBC was performed in the transfusion tube. The separating results of 18 marrow grafts showed that the recovery rates of mononuclear cells and CD34(+) cells were (83.8 +/- 55.2)% and (90.3 +/- 7.2)%, respectively. RBC residual rate was (4.3 +/- 1.5)%. The yield of CFU-GM was (60.8 +/- 22.4)/2 x 10(5) MNC, and there was no difference to [(69.8 +/- 23.4)/2 x 10(5) MNC] yielded from same marrow samples, separated by Ficoll-Hypaque separation. It is concluded that this method could be used for bone marrow transplantation.
