Accelerating hematopoietic recovery in allogeneic bone marrow transplantation mice by co-infusion of marrow stromal cells transduced with dual genes of IL-3/IL-6.
- Author:
Ji-Yang JIANG
1
;
Yong-Zhu JIN
;
Jie HAO
;
Qing-Yin ZHANG
;
Shu-Sheng XIE
Author Information
1. Department of Immunology, Peking University Health Science Center, Beijing 100083, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Bone Marrow Cells;
physiology;
Bone Marrow Transplantation;
methods;
Female;
Genetic Therapy;
Hematopoiesis;
Hematopoietic Stem Cells;
physiology;
Interleukin-3;
genetics;
Interleukin-6;
genetics;
Mice;
Mice, Inbred BALB C;
Mice, Inbred C57BL;
Stromal Cells;
physiology;
transplantation;
Transduction, Genetic
- From:
Journal of Experimental Hematology
2002;10(5):377-382
- CountryChina
- Language:Chinese
-
Abstract:
To observe whether bone marrow stromal cell line QXMSC1 (H-2(d)) engineered to secrete IL-3 and IL-6 can improved the hematopoiesis in allogeneic bone marrow transplantation (allo-BMT) mice, the stromal cell line QXMSC1IL-3/IL-6 was established by QXMSC1 cells transduced with the recombined retrovirus vector pL3SN containing mouse IL-3 cDNA and pL6SN containing human IL-6 cDNA. The lethally irradiated C57BL/6 (H-2(b)) mice were engrafted with bone marrow cells (1 x 10(7) cells/mouse BALB/c mice, H-2(d)) in which T cells were depleted by anti-Thy1.2 monoclonal antibody adding complement, and QXMSC1IL-3/IL-6 cells (5 x 10(5)/mouse) were co-infused at same time. The hematopoiesis of recipient mice was observed in 20 and 40 days post-transplantation. Blood RBC and WBC were counted, and nucleated cells, CFU-S, CFU-GM, CFU-E and CFU-GEMM were assayed in recipient bone marrow. Results showed that IL-3 and IL-6 were stably expressed in QXMSCQIL-3/IL-6 cells. Compared with BMT and co-infusion with QXMSC1 or QXMSC1 pLXSN cell groups, co-graft with QXMSC1IL-3/IL-6 cells increased the number of blood RBC and WBC in the recipients, and also significantly increased nucleated cells, CFU-S, CFU-GM, CFU-E and CFU-GEMM in recipient bone marrow. It is concluded that the marrow stromal cells transduced with IL-3/IL-6 cDNA improve the hematopoiesis in allo-BMT mice. Co-graft with QXMSC1IL-3/IL-6 cells has synergistic effect in accelerating hematopoietic reconstitution.
