Study on telomerase activity and expression of hTERT, c-myc and bcl-2 during terminal differentiation of HL-60 cells induced by retinoic acid.
- Author:
Li-De WU
1
;
Yuan-Zhong CHEN
;
Nai-Nong LI
;
Yong WU
Author Information
1. Fujian Institute of Hematology, Union Hospital of Fujian Medical University, Fuzhou 350001, China.
- Publication Type:Journal Article
- MeSH:
Cell Differentiation;
DNA-Binding Proteins;
Gene Expression Regulation, Neoplastic;
Genes, bcl-2;
Genes, myc;
HL-60 Cells;
Humans;
RNA, Messenger;
analysis;
Telomerase;
genetics;
metabolism;
Tretinoin;
pharmacology
- From:
Journal of Experimental Hematology
2002;10(5):395-399
- CountryChina
- Language:Chinese
-
Abstract:
The study was to explore the telomerase activity and the expression of hTERT, c-myc and bcl-2 mRNA during terminal differentiation of HL-60 cells induced by all trans-retinoic acid (ATRA) and to study the possible molecular mechanism. By use of the model of differentiated HL-60 cells induced by ATRA, the telomerase activity was determined by TRAP-PCR-ELISA and the expression of hTERT, c-myc, bcl-2 mRNA was detected by RT-PCR in differentiated HL-60 cells. The results showed that during differentiation of HL-60 cells, the telomerase activity was decreased, the expression of hTERT, c-myc and bcl-2 mRNA was downregulated, and the downregulation of hTERT occurred prior to suppression of telomerase activity. It is concluded that the telomerase activity is related to decrease expression of hTERT, c-myc and bcl-2 mRNA during HL-60 cell differentiation induced by ATRA.
