Inhibition of the activation of transcriptional factor NFkappaB during sodium selenite-induced apoptosis in NB4 cells.
- Author:
Jian LI
1
;
Lu ZUO
;
Ti SHEN
;
Zhi-Nan ZHANG
Author Information
1. Department of Hematology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing 100730, China.
- Publication Type:Journal Article
- MeSH:
Apoptosis;
drug effects;
Cell Division;
drug effects;
Dose-Response Relationship, Drug;
Enzyme Activation;
drug effects;
Humans;
NF-kappa B;
antagonists & inhibitors;
metabolism;
Sodium Selenite;
pharmacology
- From:
Journal of Experimental Hematology
2002;10(5):409-412
- CountryChina
- Language:Chinese
-
Abstract:
In order to evaluate the effect of sodium selenite on the activation of NFkappaB during selenite-induced apoptosis in NB4 cells, Western blot was used to measure the level of P65 in nuclear extraction of NB4 cells treated with sodium selenite to reflect the activation of NFkappaB; the apoptosis of NB4 cells was determined by morphological observation, DNA ladder electrophoresis and flow cytometry; and MTT test was used to measure the growth inhibition of cells. Results showed that sodium selenite (>/=5 micro mol/L) suppressed the cell growth, induced apoptosis and inhibited the activation of NF kappaB in a concentration- and time-dependency pattern. It was concluded that inhibition of NF kappaB might be one of the mechanisms in selenite-induced apoptosis in NB4 cells.
