Development of MTS/pms colorimetric assay in the proliferation of leukemic cells.
- Author:
Xiu-Sheng CHEN
1
;
Tie-Lan FANG
;
Rui-Bo CAI
;
Gui-Lan GUO
Author Information
1. Research Center of Gerontology, Shanxi Provincial People's Hospital, Xi'an 710068, China. lac65126@public.xa.sn.cn
- Publication Type:Journal Article
- MeSH:
Cell Division;
Colorimetry;
methods;
Formazans;
metabolism;
HL-60 Cells;
Humans;
K562 Cells;
Leukemia;
pathology;
Methylphenazonium Methosulfate;
metabolism;
Tetrazolium Salts;
metabolism;
Thiazoles;
metabolism
- From:
Journal of Experimental Hematology
2002;10(5):438-440
- CountryChina
- Language:Chinese
-
Abstract:
In order to establish a new more rapid, safe and sensitive colorimetric assay for the proliferation of leukemic cells, MTS/pms has been developed. This automated colorimetric assay is based on the characteristic of viable and metabolically active leukemic cells to cleave MTS/pms into a water-soluble product whose optical density is determined at 492 nm by an automated microtiter-plate reader photometer. The results indicated that only active leukemic cells cleaved MTS/pms into product measured, and dead cells did not reduce MTS/pms. A linear relations hip were found between the viable cell number and optical density of MTS/pms cleaved by HL-60 and K562 cell (r = 0.963). Compared with MTT and INT assays, the reduced product of MTS/pms is water-soluble. It is concluded that MTS/pms colorimetric assay is more rapid, accurate and sensitive for the bioassay of proliferation of leukemic cells.
