Activation of random-donor platelet concentrates and platelet prepared from random-donor apheresis collections during storage.
- Author:
Jun-Jie LIN
1
;
Hua-Hua FAN
;
Qing MA
;
Ming-Liang FENG
;
Xing-Feng SHEN
Author Information
1. Shanghai Blood Center, Shanghai 200051, China. linzhou69@yahoo.com.cn
- Publication Type:Journal Article
- MeSH:
Blood Donors;
Blood Preservation;
Humans;
P-Selectin;
blood;
Platelet Activation;
Platelet Membrane Glycoprotein IIb;
blood;
Plateletpheresis
- From:
Journal of Experimental Hematology
2002;10(5):458-461
- CountryChina
- Language:Chinese
-
Abstract:
The activation of random-donor platelet concentrates and platelets prepared from random-donor apheresis collections (plateletpheresis) during storage were studied. Percentage of CD62p staining and mean channel fluorescence (MCF) of CD41 of two kinds of platelets during storage on day 0, day 1, day 3 and day 5 were determined by flow cytometry. The results showed that percentages of CD62p staining and MCF of CD41 in plateletpheresis were (18.91 +/- 6.25)%, (19.48 +/- 8.27)%, (22.82 +/- 6.06)%, (56.71 +/- 11.79)% and (8.09 +/- 2.38)%, (8.13 +/- 2.45)%, (8.44 +/- 2.51)%, (19.87 +/- 6.13)%, while the results of platelet concentrates were (30.65 +/- 12.33)%, (31.46 +/- 11.86)%, (32.51 +/- 13.05)%, (63.55 +/- 13.27)% and (10.33 +/- 4.37)%, (11.09 +/- 6.61)%, (13.46 +/- 9.69)%, (24.41 +/- 10.15)%, respectively. The platelet count and pH value were also determined. The platelet number, pH value, percentage of CD62p staining and MCF of CD41 had no significant difference within 3 days of platelet storage. The platelet number and pH value decreased significantly (P < 0.001), while percentages of CD62p staining and MCF of CD41 increased significantly (P < 0.001) on day 5 of storage. It is concluded that the quality of plateletpheresis is better than platelet concentrate.
