Functional expression of Flt3 and c-kit on cord blood CD34(+) stem/progenitor cells and its significance.
- Author:
Yan-Ping MA
1
;
Ping ZOU
;
Juan XIAO
;
Shi-Ang HUANG
Author Information
1. Institute of Hematology, Union Hospital Affiliated to Tongji Medical college, Huazhong University of Science and Technology, Wuhan 430022, China. myanp@21cn.com
- Publication Type:Journal Article
- MeSH:
Antigens, CD34;
blood;
Cells, Cultured;
Fetal Blood;
cytology;
Flow Cytometry;
Hematopoietic Stem Cells;
chemistry;
Humans;
Proto-Oncogene Proteins;
analysis;
genetics;
physiology;
Proto-Oncogene Proteins c-kit;
analysis;
genetics;
physiology;
RNA, Messenger;
analysis;
Receptor Protein-Tyrosine Kinases;
analysis;
genetics;
physiology;
Reverse Transcriptase Polymerase Chain Reaction;
Stem Cell Factor;
pharmacology;
fms-Like Tyrosine Kinase 3
- From:
Journal of Experimental Hematology
2002;10(4):277-280
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the expression and function of the receptors of early-acting cytokines on cord blood CD34(+) hematopoietic stem/progenitor cells, the studies of Flt3 and c-kit were undertaken at the gene and protein levels. Fresh and cultured cord blood CD34(+) stem/progenitor cells were analyzed by flow cytometric two-color direct immunofluorescence methods and RT-PCR, while function of receptors was studied in vitro. It was found that (68.8 +/- 15.4)% of CD34(+) cells expressed Flt3, (50.6 +/- 12.7)% of CD34(+) cells expressed c-kit, the proportion of CD34(+) cells expressing Flt3 and c-kit decreased as in vitro culture time extended. It was concluded that cord blood CD34(+) stem/progenitor cells are capable of expressing Flt3 and c-kit for as long as 2 - 3 weeks in liquid medium, during the first week of culture, SCF and FL enhanced the generation of cells and progenitors notably.
