Mesenchymal stem cells suppress allogeneic T cell responses by secretion of TGF-beta1.
- Author:
Jian-Lin CHEN
1
;
Zi-Kuan GUO
;
Chen XU
;
Yu-Hang LI
;
Chun-Mei HOU
;
Ning MAO
;
Hu CHEN
Author Information
1. Center of Hematopoietic Stem Cell Transplantation, Hospital Affiliated to Academy of Military Medical Sciences, Beijing 100039, China. JianLin-Chen@hotmail.com
- Publication Type:Journal Article
- MeSH:
Bone Marrow Cells;
physiology;
Bone Marrow Transplantation;
immunology;
Enzyme-Linked Immunosorbent Assay;
Flow Cytometry;
Humans;
Isoantigens;
immunology;
Lymphocyte Culture Test, Mixed;
Reverse Transcriptase Polymerase Chain Reaction;
Stem Cells;
physiology;
secretion;
T-Lymphocytes;
immunology;
Transforming Growth Factor beta;
physiology;
Transforming Growth Factor beta1
- From:
Journal of Experimental Hematology
2002;10(4):285-288
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the mechanisms underlying the issue that bone marrow mesenchymal stem cells (MSC) could trigger low responsiveness of allogeneic T lymphocytes against alloantigens, phenotypes of T cells were analysed with flow cytometry before and after coculture of allogeneic T lymphocytes with MSC. Further, expression of cytokines including IL-4, IFN-gamma and TGF-beta1 was evaluated by RT-PCR and ELISA as well. The results showed that CD8(+) subpopulation was increased and CD25(+) subset was decreased in proportion after coculture of allogeneic T lymphocytes with MSC for 2 weeks. RT-PCR assay showed that MSC expressed TGF-beta1 but not IL-4 and IFN-gamma, and the result was further proved by ELISA assay showing that the secretion of TGF-beta1 reached to 1 ng/ml in 72 hours. It was concluded that allogeneic MSC suppressed T cells activation by alteration of T cell subpopulations. The suppressive effect was at least in part due to the secretion of TGF-beta1. The results indicate that MSC pretreatment might be useful in the prevention of GVHD in HLA-mismatched bone marrow transplantation and further donors for hematopoietic stem cells could be selected from greater potentials.
