Study on expression of cytokines mRNA induced by B7-1-transfected Raji and Jurkat cells.
- Author:
Li-Ping JIA
1
;
Xiao-Yan KE
Author Information
1. Department of Hematology, The Third Clinical Hospital, Peking University, Beijing 100083, China.
- Publication Type:Journal Article
- MeSH:
B7-1 Antigen;
genetics;
physiology;
Cytokines;
genetics;
Humans;
Interferon-gamma;
genetics;
Interleukin-2;
genetics;
Interleukin-4;
genetics;
Jurkat Cells;
RNA, Messenger;
analysis;
Transfection
- From:
Journal of Experimental Hematology
2002;10(4):322-326
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the function of B7 co-stimulator in activation and differentiation of T cell, B7 gene was transfected into Raji and Jurkat cells by liposome, B7 expression in tumor cells was detected with flow cytometry, and expression of IL-2, IL-4 and IFN-gammamRNA was detected by RT-PCR. Kinetics of secretion of three cytokines was also analyzed at 4, 12, 20 and 48 hours after gene transfection. The results showed that B7(+) Raji cells could induce mRNA expression of IL-2, IL-4 and IFN-gamma on T cell surface; B7(+) Jurkat cells could induce secretion of IL-2 and IFN-gamma. However, B7(-) Raji and B7(-) Jurkat cells could not induce secretion of cytokines. Kinetics of the three cytokines secretion were different, IL-2 and IL-4 were only detectable after 4 hours of T cell activation, whereas IFN-c was detectable after 12 hours of stimulation. The peak levels of IL-2, IL-4 and IFN-gamma were found at 20 hours after activation. It was concluded that tumor cell lines transfected with B7 gene could enhance their immunocompetence, activating T cell efficiently and B7-1 play more critical role in T cell activation and differentiation.
