CD62p expression in platelet during the preparation course of Cryopreservated platelet-rich plasma.
- Author:
Jing-Han LIU
1
;
Xi-Lin OUYANG
;
Qun SHI
;
Qun LUO
;
Xi-Jin LI
;
Hai-Bao WANG
;
Min-Cai CHENG
;
Wei HAN
;
Dayong GAO
Author Information
1. Department of Blood Transfusion, The General Hospital of PLA, Centre for Clinical Blood Transfusion Medicine of PLA, Beijing 100853, China.
- Publication Type:Journal Article
- MeSH:
Blood Platelets;
metabolism;
Blood Preservation;
methods;
standards;
Cryopreservation;
methods;
standards;
Flow Cytometry;
Humans;
P-Selectin;
biosynthesis
- From:
Journal of Experimental Hematology
2002;10(3):253-256
- CountryChina
- Language:Chinese
-
Abstract:
In order to explore the factors that affect CD62p expression in platelet during the whole course of cryopreservated platelets preparation, CD62p expression of platelet was evaluated by flow cytometry assay. The whole course of cryopreservated platelets preparation in order included whole blood collection, centrifugation for fresh platelet-rich plasma preparation, addition of dimethyl sulfoxide, and freeze in -80 degrees C refrigerator and thaw in 38 degrees C water bath. Result showed that the CD62p expressed slowly from whole blood collection to addition of dimethyl sulfoxide, but expressed abruptly after freeze and thaw and it occupied 82 per cent of the whole expression. It was concluded that the whole blood collection, centrifugation for fresh platelet enriched plasma preparation and addition of dimethyl sulfoxide were optimized in the whole course, but the damage to platelets in the whole course of cryopreservation could not be avoided. It suggests that improved techniques are needed to reduce the damage to cryopreservated platelets.
