Effects of panax notoginseng saponins on expression of intercellular adhesion molecule-1 in human umbilical vein endothelial cells injury induced by oxidized low-density lipoprotein.
- Author:
Jian-hui QIN
1
;
Ling-qun ZHU
;
Wei CUI
Author Information
- Publication Type:Journal Article
- MeSH: Arteriosclerosis; drug therapy; metabolism; physiopathology; Cell Adhesion; drug effects; Cells, Cultured; Drugs, Chinese Herbal; pharmacology; Endothelium, Vascular; cytology; drug effects; metabolism; Gene Expression; drug effects; Humans; Intercellular Adhesion Molecule-1; genetics; metabolism; Lipoproteins, LDL; metabolism; Panax notoginseng; chemistry; Umbilical Veins; cytology; drug effects; metabolism
- From: Chinese Journal of Integrated Traditional and Western Medicine 2008;28(12):1096-1099
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the protective effect of panax notoginseng saponins (PNS) on human umbilical vascular endothelial cells (HUVECs) injury induced by oxidized low-density lipoprotein (ox-LDL) for investigating the mechanism of PNS in treating arteriosclerosis obliterans (ASO).
METHODSTaking the cultured HUVECs as target cells, ox-LDL was used to establish a model of injured HUVEC and it was then intervened by PNS. The morphologic changes of HUVEC were observed under light microscope; activity of cells was determined by MTT method; the adhesive percentage between ox-LDL treated HUVEC and monocyte detennined by protein quantification and the protein expression of intercellular adhesion molecule-1 (ICAM-1) determined by flow cytometry.
RESULTSAt the time points of HUVEC being treated with ox-LDL (100 mg/L) for 12 h and 24 h, significant injury of HUVEC was shown, its activity reduced, the adhesion rate with monocytes elevated, and the protein expression of ICAM-l in HUVEC increased significantly (P < 0.05, P < 0.01). PNS showed significant effect in reversing all the above changes, as compared with the control group (without PNS treaded), respective significant difference was shown in all the four indexes (P < 0.05, P < 0.01).
CONCLUSIONPNS has a protective effect on endothelial cells injury induced by ox-LDL,which may be one of its mechanisms in treating ASO. The protective effect of PNS is probably by way of down-regulating the expression of ICAM-1 in endothelial cells and inhibiting the adherence of monocytes to endothelial cells.