Selective effect of nispex in inhibiting human cancer cell proliferation and inducing cell apoptosis.
- Author:
Yi-Jun XIAO
1
;
Yuan-Zhong CHEN
;
Bing-Hua CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antineoplastic Agents, Phytogenic; isolation & purification; pharmacology; Apoptosis; drug effects; Cell Proliferation; drug effects; Drugs, Chinese Herbal; chemistry; isolation & purification; Flavonoids; isolation & purification; pharmacology; Humans; Loranthaceae; chemistry; Neoplasms; pathology; Rats; Species Specificity; Tumor Cells, Cultured
- From: Chinese Journal of Integrated Traditional and Western Medicine 2009;29(2):148-152
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the in vitro anticancer effect of Nispex, the total flavonoids extract from Scurrula parasitic L.
METHODSThe cell proliferation inhibitory effects of Nispex on various kinds of tumor cells or non-tumor cells in human and rats were detected with MTT assay and colony forming assay respectively, the cell apoptosis induced by Nispex was detected by AO/EB fluorescence staining, TUNEL assay and AnnexinV-FITC/PI flow cytometry.
RESULTSNispex could significantly inhibit human cancer cell proliferation and induce human cancer cell apoptosis, especially to the proliferative cell group, but its inhibition on human non-tumor cell was insignificant, and showed no effect on murine cancer cells in the tested scope.
CONCLUSIONNispex is a nature plant extract which shows good selectivity for killing human cancer cell.
