Immunological comparison of Efb and ClfA of Staphylococcus aureus isolated from bovine.
- Author:
Yuntao LIU
;
Yan SUL
;
Baojiang ZHANG
;
Lingling SU
;
Huijiao JIANG
- Publication Type:Journal Article
- MeSH:
Animals;
Antibodies, Bacterial;
blood;
Bacterial Adhesion;
Bacterial Proteins;
immunology;
Cattle;
microbiology;
Coagulase;
immunology;
Enzyme-Linked Immunosorbent Assay;
Fibrinogen;
metabolism;
Genetic Vectors;
Immune Sera;
immunology;
Immunization;
Rabbits;
Staphylococcal Infections;
immunology;
Staphylococcus aureus
- From:
Chinese Journal of Biotechnology
2015;31(9):1335-1343
- CountryChina
- Language:Chinese
-
Abstract:
To compare immunological characteristics of Extracellular fibrinogen-binding protein (Efb) and Clumping factor A (CfA) of Staphylococcus aureus, we constructed two prokaryotic expression vector pET28a-Efb and pET28a-ClfA. After prokaryotical expression and purification, Efb and ClfA were used to immunize experimental animal. After the second immunization the antisera were collected and the antibody titers, the bacteria binding activity and adhesion inhibition activity of these antisera were detected by enzyme linked immunosorbent assay, adhesion inhibition assay and challenge. Both Efb and ClfA had Fibrinogen binding activity whereas the former had better Fibronectin binding activity. The bacteria binding capability of antisera of rabbits immunized with ClfA was better than that with Efb (P < 0.01). Both antisera of Efb and ClfA could inhibit adherence activity of Staphylococcus aureus to Fibrinogen and Fibronectin adherence compare to the control group (P < 0.01), and Efb had better adhesion inhibition activity than ClfA. The antibody titer of immunized group could reach 1:40 500. After the second immunization, both Efb and ClfA had good protective efficacy. This result constitutes a good foundation for Staphylococcus aureus subunit vaccine development.
