Overexpression of FKS1 to improve yeast autolysis-stress.
- Author:
Jia LI
;
Jinjing WANG
;
Qi LI
- Publication Type:Journal Article
- MeSH:
Autolysis;
Beer;
Echinocandins;
genetics;
Glucosyltransferases;
genetics;
Hypergravity;
Membrane Proteins;
genetics;
Saccharomyces cerevisiae;
cytology;
Saccharomyces cerevisiae Proteins;
genetics
- From:
Chinese Journal of Biotechnology
2015;31(9):1344-1354
- CountryChina
- Language:Chinese
-
Abstract:
With the development of high gravity brewing, yeast cells are exposed to multiple brewing-associated stresses, such as increased osmotic pressure, enhanced alcohol concentration and nutritional imbalance. These will speed up yeast autolysis, which seriously influence beer flavor and quality. To increase yeast anti-autolytic ability, FKS1 overexpression strain was constructed by 18S rDNA. The concentration of β-1,3-glucan of overexpression strain was 62% higher than that of wild type strain. Meantime, FKS1 overexpression strain increased anti-stress ability at 8% ethanol, 0.4 mol/L NaCl and starvation stress. Under simulated autolysis, FKS1 showed good anti-autolytic ability by slower autolysis. These results confirms the potential of FKS1 overexpression to tackle yeast autolysis in high-gravity brewing.
