Breeding of high-producing LI-F lipopeptide Paenibacillus polymyxa by protoplast fusion and differential expression analysis of fusion strains.
- Author:
Dong YAN
;
Jinzhi HAN
;
Xiaomei BIE
;
Zhaoxin LU
;
Fengxia LÜ
;
Haizhen ZHAO
;
Chong ZHANG
- Publication Type:Journal Article
- MeSH:
Anti-Bacterial Agents;
biosynthesis;
Chromatography, High Pressure Liquid;
Lipopeptides;
biosynthesis;
Paenibacillus;
metabolism;
Protoplasts;
metabolism;
Real-Time Polymerase Chain Reaction
- From:
Chinese Journal of Biotechnology
2015;31(9):1401-1407
- CountryChina
- Language:Chinese
-
Abstract:
Auxotrophic strains of N1-37 (Phe-) and N2-27 (His-), screened from mutations of Paenibacillus polymyxa JSa-9 previously, were used as the parent strains to screen high-producing LI-F antibacterial lipopeptide fusion strain through protoplast fusion with polyethylene glycol as a promote agent. Fusion strain F5-15 was obtained. Then the product of LI-F antibacterial lipopeptide was quantified by HPLC, and the difference of expression of the key genes of lipopeptide synthase between wild strain JSa-9 and the fusion strain was analyzed by real-time PCR. LI-F antibacterial lipopeptide yield of the fusion strain F5-15 was 3.1-fold of the original strain JSa9's, and the expression levels of the target genes were 10.48, 2.48, 2.1 and 11.8 fold of the initial strain JSa-9, respectively.
