Automatically feeding strategy for 1,3-propanediol fermentation of Klebsiella pneumoniae LDH526.
- Author:
Jinhai HUANG
;
Zhen CHEN
;
Yan SUN
;
Dehua LIU
- Publication Type:Journal Article
- MeSH:
Culture Media;
Fermentation;
Glycerol;
Industrial Microbiology;
methods;
Klebsiella pneumoniae;
growth & development;
Propylene Glycol;
Propylene Glycols;
metabolism
- From:
Chinese Journal of Biotechnology
2015;31(10):1520-1527
- CountryChina
- Language:Chinese
-
Abstract:
1,3-propanediol is an important chemical used as building block for the synthesis of highly promising polyesters such as polytrimethylene terephthalate. A genetically modified Klebsiella pneumoniae LDH526 can use glycerol as sole carbon source and produce 1,3-propanediol with the titer above 90 g/L. A key factor affecting the production of 1,3-propanediol by the mutant K. pneumoniae is the accurate control of the feeding of glycerol. To generate a robust and reproducible fermentation process of 1,3-propanediol, we designed and optimized an automatically feeding strategy of glycerol based on fermentation kinetics. By coupling the substrate feeding rate with easily observed variables -pH and fermentation time, we have achieved self-starting glycerol feeding and dynamic control of the glycerol concentration during the fermentation process. This automated system allowed us to generate a reproducible, consistent and operator-independent process from lab-scale to production scale. The final concentration of 1,3-propanediol was above 95 g/L after 72 h.
