A nonsurgical embryo transfer technique in mice.

Xinchong Duan; Yang LI; Keqian DI; Yong Huang; Xiangyun LI

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A nonsurgical embryo transfer technique in mice.

Author: Xinchong DUAN ¹ ; Yang LI ¹ ; Keqian DI ² ; Yong HUANG ³ ; Xiangyun LI ¹
Author Information

1. College of Animal Science and Technology, Hebei Agricultural University, Baoding 071001, Hebei, China.
2. Centre of Laboratory Animal Faculty of Medicine, Hebei University, Baoding 071001, Hebei, China.
3. Institute of Animal Husbandry Engineering, Chongqing Academy of Animal Sciences, Chongqing 402460, China.
Publication Type:Journal Article
Keywords: blastocysts; embryo lost; mouse; nonsurgical embryo transfer; uterine injury
MeSH: Animals; Blastocyst; Embryo Transfer; methods; veterinary; Female; Mice; Pregnancy
From: Chinese Journal of Biotechnology 2016;32(4):440-446
CountryChina
Language:Chinese
Abstract: Although efficient nonsurgical transfer of embryos in mice would provide many advantages over a surgical method, the low success rate of nonsurgical transfer has hampered its acceptance and use. Here, a plastic catheter was used to mimic embryo transfer process and then the transfer efficiency was evaluated by intrauterine trypan blue dye dispersion. Also 3.5-day blastocysts from natural pregnant mice were transferred through cervix into uterine horns. The results show that 70.9% of CD-1 mouse 3.5-day blastocysts transferred into unilateral uterine horns of pseudopregnant 2.5-day recipients can be developed to live newborns, and an efficient mouse nonsurgical embryo transfer technique was established. The technique was simple, rapid, inexpensive, unlikely to get contaminated, ethical and do not need specialized apparatus, and can completely replace surgical embryo transfer techniques. Moreover, the mouse nonsurgical embryo transfer technique provides a research model for human and other large animal embryo transfer.