Research progress in hydroxylase in taxol biosynthetic pathway.

Author: Qingpu CHEN ¹ ; Weifang LIAO ¹ ; Chunhua FU ¹ ; Chunfang ZHAO ¹ ; Longjiang YU ¹
Author Information

1. Department of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, Hubei, China.
Publication Type:Journal Article
Keywords: biosynthesis; hydroxylase; research progress; taxol
MeSH: Biosynthetic Pathways; Mixed Function Oxygenases; metabolism; Paclitaxel; biosynthesis; Synthetic Biology; Taxus; enzymology
From: Chinese Journal of Biotechnology 2016;32(5):554-564
CountryChina
Language:Chinese
Abstract: Taxol is a secondary metabolite with prominent anti-tumor activity, but the yield cannot meet the growing clinical demand due to lower content in yew. Now, most enzyme genes involved in taxol biosynthesis have been cloned and identified, so that obtaining this drug by using synthetic biology method has become a hotspot in recent years. However, most hydroxylases involved in taxol biosynthetic pathway have not been explored. Here, we reviewed the progress on the biosynthesis pathway of taxol, especially concerning hydroxylase. The future research areas of taxol biosynthesis through synthetic biology were also discussed to provide basis for the discovery of uncharacterized hydroxylase genes and the mass taxol production by synthetic biology technology.