Prokaryotic expression and immunogenicity of IgG-binding protein of Streptococcus equi subspecies equi.

Jungao Shao; Huijiao Jiang; Jianxin Chang; Baojiang Zhang; Shanchun LI; Yan SU

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Prokaryotic expression and immunogenicity of IgG-binding protein of Streptococcus equi subspecies equi.

Author: Jungao SHAO ¹ ; Huijiao JIANG ¹ ; Jianxin CHANG ¹ ; Baojiang ZHANG ¹ ; Shanchun LI ¹ ; Yan SU ¹
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1. College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi 830052, Xinjiang, China.
Publication Type:Journal Article
Keywords: EAG protein; Streptococcus equi subspecies equi; expression; immunogenicity analysis
MeSH: Animals; Antibodies, Bacterial; blood; Antigens, Bacterial; immunology; Bacterial Proteins; immunology; Bacterial Vaccines; immunology; Immunity, Humoral; Immunoglobulin G; blood; Mice; Polymerase Chain Reaction; Protein Binding; Recombinant Proteins; immunology; Streptococcal Infections; prevention & control; Streptococcus equi; Vaccination
From: Chinese Journal of Biotechnology 2016;32(5):577-583
CountryChina
Language:Chinese
Abstract: To analyze the immunogenicity and protective ability of recombinant IgG-binding protein (EAG) of Streptococcus equi subspecies equi and to evaluate its value when used as equine vaccine antigen, EAG gene was amplified by PCR and inserted into pET-28a vector. The EAG recombinant proteins were expressed and purified to immune mice. The serum antibody and challenge protection were tested. The purified recombinant protein of EAG was 26 kDa, and the protein reacted specifically with positive serum of Streptococcus equi subspecies equi. The mice antibody level for EAG immunization group was 1∶8 100. The immunological protection result showed that the protection rate of the EAG recombinant protein was 90%. The results suggested that the EAG protein has good immunogenicity and immunological protection, and it can effectively increase the humoral immune response and immunological protection of mice.