Transcriptome analysis of Pichia pastoris in response to ethanol stress.
- Author:
Peng GAO
1
;
Jian DING
1
;
Xu ZHANG
1
;
Yue ZHAO
1
;
Meng ZHANG
1
;
Minjie GAO
1
;
Jianrong WU
1
;
Xiaobei ZHAN
1
Author Information
- Publication Type:Journal Article
- Keywords: Pichia pastoris; ethanol stress; heterologous protein; transcriptome
- MeSH: Amino Acids; metabolism; Bioreactors; Endoplasmic Reticulum Stress; Energy Metabolism; Ethanol; chemistry; Gene Expression Profiling; Gene Expression Regulation, Fungal; Glycerol; Methanol; Pichia; metabolism; Protein Biosynthesis; drug effects; Protein Folding; Recombinant Proteins; biosynthesis; Transcriptome
- From: Chinese Journal of Biotechnology 2016;32(5):584-598
- CountryChina
- Language:Chinese
- Abstract: Effective expression of pIFN-α in recombinant Pichia pastoris was conducted in a 5 L fermentor. Ethanol accumulation during the late glycerol feeding period inhibited heterologous protein expression. Comparative transcriptome analysis was thus performed to compare the gene transcription profiles of Pichia pastoris KM71H in high and low ethanol concentration environments. The results showed that during the glycerol cultivation stage, 545 genes (265 up-regulated and 280 down-regulated) were differentially expressed with ethanol stress. These genes were mainly involved in protein synthesis, energy metabolism, cell cycle and peroxisome metabolism. During the methanol induction stage, 294 genes (171 up-regulated and 123 down-regulated) were differentially expressed, which were mainly related to methanol metabolism, amino acid metabolism and protein synthesis. Ethanol stress increased protein misfolding and reduced structural integrity of ribosome and mitochondria during cultivation stage, and led to the failure of endoplasmic reticulum stress removal and damaged amino acid metabolism during induction stage in Pichia pastoris.
