Isolation, identification and structural characterization of secondary metabolites from amarine sponge-derived rare actinobacterium Dermacoccus sp. X4.
- Author:
Yanfeng ZHANG
1
;
Yong XU
1
;
Lei CHEN
1
;
Jun HU
1
;
Xuecheng ZHANG
1
;
Wei FANG
1
;
Zemin FANG
1
;
Yazhong XIAO
1
Author Information
- Publication Type:Journal Article
- Keywords: bacteriostatic activity; isolation and purification; secondary metabolite; sponge associated microorganism; structural characterization
- MeSH: Actinomycetales; chemistry; Animals; China; Chromatography, Liquid; Indoles; isolation & purification; pharmacology; Magnetic Resonance Spectroscopy; Mass Spectrometry; Porifera; microbiology; Seawater; Secondary Metabolism; Staphylococcus aureus; drug effects
- From: Chinese Journal of Biotechnology 2016;32(5):599-609
- CountryChina
- Language:Chinese
- Abstract: We isolated and identified the symbiotic and adnascent microorganisms from an unidentified sponge collected from 10-meter-deep seawater of the Paracel Islands in China. A total of 16 strains were obtained and identified. Through bacteriostatic activity assay, one of the strains, Dermacoccus sp. X4, was found to effectively inhibit the growth of Staphylococcus aureus. Subsequently, its secondary metabolites were purified by silica gel partition, octadecylsilane (ODS) reverse phase, Sephadex™LH-20 size exclusion, and C18 reverse phase chromatography. Using liquid chromatography, mass spectrometry, and nuclear magnetic resonance, three of the purified compounds were structurally characterized to be one 3-(4-hydroxybenzyl) hexahydropyrrolo [1,2-a]pyrazine-1,4-dione and two indole acid glycerides. This is the first report about indole acid glyceride isolated from microbial secondary metabolites, enriching marine drug candidate resources.
