Structure and immunomodulatory activity of extracellular polysaccharide from Grifola frondosa.
- Author:
Lirong HAN
1
;
Dai CHENG
1
;
Lirui WANG
1
;
Chunling WANG
1
Author Information
- Publication Type:Journal Article
- Keywords: Grifola frondosa polysaccharide; RAW264.7 cells; immunomodulatory activity; structural analysis
- MeSH: Animals; Cytokines; metabolism; Grifola; chemistry; Mice; Nitric Oxide Synthase Type II; metabolism; Polysaccharides; immunology; RAW 264.7 Cells
- From: Chinese Journal of Biotechnology 2016;32(5):648-656
- CountryChina
- Language:Chinese
- Abstract: We aimed at analyzing the structure of extracellular polysaccharide A from Grifola frondosa (EXGFP-A) and testing its immunomodulatory activity. Structural analysis shows that EXGFP-A was a contained α-D-glucoside bond and pyranose ring. GC analysis reveals that EXGFP-A was mainly composed of rhamnose, arabinose, xylose, mannose, glucose, galactose, by the molar ratio of 0.28:0.31:0.30:0.06:7.98:0.61. The results of MTT(3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay indicates when EXGFP-A was at a concentration of 80 μg/mL and treatment time of 48 h, RAW264.7 cells proliferation index reached a maximum of 137.5%. Meanwhile, the AO staining showed that EXGFP-A activated RAW264.7 cells and improved the level of intracellular nucleic acid metabolism. In addition, in a certain range of concentration, EXGFP-A was able to increase the release of NO in RAW264.7 cells, and upregulate the mRNA expression of immunological factor TNF-α, IL-1β, IL-6, IL-12, IFN-γ and iNOS of RAW264.7 cells. Our results confirm that EXGFP-A had immunomodulatory activity. Our findings provided scientific basis for the structural analysis and application of Grifola frondosa polysaccharide.
