Application of optimized multi-enzyme combination and sample pretreatment in proteomics.
- Author:
Feilin WU
;
Mingzhi ZHAO
;
Zhi XIONG
;
Ping XU
- Publication Type:Journal Article
- MeSH:
Enzymes;
chemistry;
Mass Spectrometry;
Proteins;
chemistry;
Proteomics;
methods;
Trypsin;
chemistry
- From:
Chinese Journal of Biotechnology
2016;32(3):306-316
- CountryChina
- Language:Chinese
-
Abstract:
Proteomics is a powerful subject focusing on large-scale study of protein structures and functions. A complete enzymatic digestion of protein complexes is the key step in modern high-resolution and high-throughput mass spectrometry (MS)-based identification and quantification. To achieve MS analysis, both peptide sample pretreatment and data acquisition are prerequisite in proteomic studies. In this paper, we summarized both the enzymatic proprieties of three common proteolytic enzymes, Trypsin, Lys-C and Glu-C, the optimization of multi-enzyme combination and an advanced sample pretreatment in proteomics research.
