Effect of Tiam-l gene silencing on human colorectal carcinoma cell line SW480 metastasis in nude mice observed by real-time whole-body fluorescence imaging.
- Author:
Juan-zhi CHEN
1
;
Yong-jian DENG
;
Si-ming XIE
;
Yan-qing DING
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Blotting, Western; Bone Neoplasms; genetics; metabolism; secondary; Cell Line, Tumor; Cell Survival; Colorectal Neoplasms; genetics; metabolism; pathology; Diagnostic Imaging; methods; Female; Fluorescence; Gene Silencing; Green Fluorescent Proteins; chemistry; genetics; metabolism; Guanine Nucleotide Exchange Factors; genetics; metabolism; Immunohistochemistry; Liver Neoplasms; genetics; metabolism; secondary; Lung Neoplasms; genetics; metabolism; secondary; Mice; Mice, Nude; Microscopy, Fluorescence; Neoplasm Transplantation; T-Lymphoma Invasion and Metastasis-inducing Protein 1; Transplantation, Heterologous
- From: Journal of Southern Medical University 2007;27(6):756-759
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the effect of Tiam-l gene silencing on the metastasis of human colorectal carcinoma cell line SW480 in nude mice by real-time whole-body fluorescence imaging.
METHODSEnhanced green fluorescence protein (EGFP)-labeled human colorectal carcinoma cells, SW480/EGFP(+)/Tiam-1(-) and SW480/EGFP(+), were implanted into nude mice via tail vein injection or orthotopic colonal inoculation, and real-time whole-body fluorescence imaging was performed to compare the difference in tumor progression and metastasis between the two cells.
RESULTSBoth SW480/EGFP(+) and SW480/ EGFP(+)/Tiam-1(-) cells stably expressed EGFP, and Tiam1 gene expression was reduced in SW480/EGFP(+)Tiam-1(-) to 30% of the expression level in SW480/EGFP(+) cells. The growth rate of the two cell lines had no significant difference in vitro (P>0.05), but SW480/EGFP(+)/Tiam1(-) cell proliferation and metastasis were depressed obviously in comparison with SW480/EGFP(+) in vivo (P<0.05).
CONCLUSIONTiam-1 gene may play an important role in invasion and metastasis of human colorectal cancer.
