Microsatellite analysis and hMLH1/hMSH2 expression detection in young patients with colorectal cancer: value in screening hereditary nonpolyposis colorectal cancer.
- Author:
Lei YANG
1
;
Yan-qing DING
;
Guo-xin LI
;
Jiang YU
;
Yu WANG
;
Jun ZHOU
;
Hong-jun YANG
;
Jin-hua ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Adaptor Proteins, Signal Transducing; biosynthesis; Adolescent; Adult; Colorectal Neoplasms; genetics; metabolism; pathology; Colorectal Neoplasms, Hereditary Nonpolyposis; diagnosis; genetics; metabolism; Female; Humans; Immunohistochemistry; Male; Mass Screening; Microsatellite Instability; Microsatellite Repeats; genetics; MutL Protein Homolog 1; MutS Homolog 2 Protein; biosynthesis; Nuclear Proteins; biosynthesis; Young Adult
- From: Journal of Southern Medical University 2007;27(6):779-782
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate the frequency of microsatellite instability (MSI) and absence of hMLH1/hMSH2 expression in young patients with colorectal cancer, and investigate their role in screening hereditary nonpolyposis colorectal cancer (HNPCC).
METHODSeventy-three young patients (below 40 years old) with colorectal cancer were examined for DNA mismatch repair deficiency by microsatellite testing and immunohistochemical detection of hMLH1/hMSH2 gene products.
RESULTSThe frequency of MSI and absence rate of hMLH1/hMSH2 expression are 56.16% and 49.32% in these patients, respectively, which increased significantly with younger age for cancer diagnosis.
CONCLUSIONDefects in the DNA mismatch repair system are frequent in Chinese young patients with colorectal cancer. Microsatellite analysis and immunohistochemical detection are useful for efficient identification of HNPCC in these young patients.
