Effect of Leonurus Heterophyllus Sweet on tissue factor transcription and expression in human umbilical vein endothelial cells in vitro.
- Author:
Lian ZHENG
1
;
Chi-hua FANG
Author Information
- Publication Type:Journal Article
- MeSH: Cells, Cultured; Dose-Response Relationship, Drug; Drugs, Chinese Herbal; pharmacology; Endothelial Cells; cytology; drug effects; metabolism; Gene Expression; genetics; Humans; Leonurus; chemistry; Reverse Transcriptase Polymerase Chain Reaction; Thromboplastin; genetics; Time Factors; Umbilical Veins; cytology
- From: Journal of Southern Medical University 2007;27(6):805-808
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of Leonurus Heterophyllus Sweet, (LHS) on tissue factor (TF) transcription and expression induced by thrombin in human umbilical vein endothelial cells (HUVECs).
METHODSHUVECs were incubated with different concentrations of LHS and the TF mRNA expression was detected by reverse transcript-polymerase chain reaction (RT-PCR).
RESULTSLHS treatment of HUVECs at different concentrations and for different times resulted in significant differences in TF expression (Plt;0.01). The transcription of TF in LHS-treated cells was significantly different from that of the blank control group (Plt;0.01).
CONCLUSIONLHS can decrease the expression of TF and intervene with TF transcription in HUVECs in vitro.
