Cloning and functional study of a novel aromatic-ring-hydroxylating dioxygenase gene.
- Author:
Hong-wei ZHOU
1
;
Mei-juan ZHOU
Author Information
1. Department of Enviromental health, School of Public Heath anf Tropical Medicine, Southern Medical University, Guangzhou, China. hzhou@fimmu.com
- Publication Type:Journal Article
- MeSH:
Actinomycetales;
enzymology;
genetics;
Bacterial Proteins;
genetics;
metabolism;
Biodegradation, Environmental;
Cloning, Molecular;
Dioxygenases;
genetics;
metabolism;
Electrophoresis, Polyacrylamide Gel;
Escherichia coli;
genetics;
metabolism;
Fluorenes;
metabolism;
Hydroxylation;
Molecular Sequence Data;
Phenanthrenes;
metabolism;
Polycyclic Aromatic Hydrocarbons;
metabolism;
Pyrenes;
metabolism;
Recombinant Proteins;
metabolism
- From:
Journal of Southern Medical University
2007;27(5):717-719
- CountryChina
- Language:English
-
Abstract:
The aromatic-ring-hydroxylating dioxygenase is a key enzyme that initiates the biodegradation of polycyclic aromatic hydrocarbons in bacteria. In the present study, a novel dioxygenase sequence was cloned from Terrabacter sp. FLO using a genome walking method. The dioxygenase was cloned into pET17 and actively expressed in E.coli BL21 (DE3) in co-expression with electron transfer chain proteins. The recombinant dioxygenase was found to transform phenanthrene, fluorene, pyrene and fluoranthene into the cis-dihydrodiol metabolites.
