Koumine-induced apoptosis of human colonic adenocarcinoma cells: the cell biological mechanism.
- Author:
De-biao CHI
1
;
Lin LI
;
Li-sha SUN
;
Wen-feng MA
Author Information
- Publication Type:Journal Article
- MeSH: Adenocarcinoma; metabolism; pathology; Antineoplastic Agents; pharmacology; Apoptosis; drug effects; Calcium; metabolism; Cell Communication; drug effects; Cell Line, Tumor; Colonic Neoplasms; metabolism; pathology; Gap Junctions; drug effects; metabolism; Humans; Indole Alkaloids; pharmacology; Membrane Potential, Mitochondrial; drug effects; Microscopy, Confocal; Reactive Oxygen Species; metabolism
- From: Journal of Southern Medical University 2007;27(7):994-997
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the cell biological mechanism of koumine-induced apoptosis of human colonic adenocarcinoma cells.
METHODSThe effects of LoVo cell koumine on the membrane potential, mitochondrial membrane potential, concentration of cytosolic free calcium, reactive oxygen species (ROS) and gap junctional intercellular communication was observed by laser scanning confocal microscopy.
RESULTS AND CONCLUSIONKoumine lowered the membrane potential and mitochondrial membrane potential of LoVo cells and decreased the concentration of free cytosolic calcium, which was increased to a level higher than the basal one after addition of EDTA. Koumine also increased the reactive oxygen species and enhanced the gap junctional intercellular communication of LoVo cells. These findings may explain the possible mechanisms of koumine-induced LoVo cell apoptosis.
