HL-60 cell apoptosis induced by bortezomib alone or in combination with arsenic trioxide in vitro.
- Author:
Qi-xin SUN
1
;
Fan-yi MENG
;
Yun-bi FU
;
Li LI
;
Shuai TIAN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Apoptosis; drug effects; Arsenicals; administration & dosage; pharmacology; Boronic Acids; administration & dosage; pharmacology; Bortezomib; Cell Proliferation; drug effects; Cell Survival; drug effects; Dose-Response Relationship, Drug; Drug Synergism; Electrophoresis; Flow Cytometry; HL-60 Cells; Humans; Oxides; administration & dosage; pharmacology; Pyrazines; administration & dosage; pharmacology; Time Factors
- From: Journal of Southern Medical University 2007;27(7):1022-1025
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of bortezomib alone and in combination with arsenic trioxide on apoptosis of HL-60 cells.
METHODSHL-60 cells were treated with bortezomib alone or in combination with arsenic trioxide for 12 to 48 h and the cell proliferation was analyzed with MTT assay, and cell apoptosis detected by DNA gel electrophoresis, fluorescence microscopy and flow cytometry.
RESULTSAt the concentrations of 10 to 50 nmol/L, bortezomib effectively inhibited HL-60 cell proliferation, and induced cell apoptosis. A 12-hour bortezomib treatment at 10 nmol/L was sufficient to induce cell apoptosis, and prolonged treatment or increased concentration significantly increased HL-60 cell apoptotic rate. Combined treatment of the cells with bortezomib (10 nmol/L) and arsenic trioxide (15 micromol/L) resulted in an even higher cell apoptosis rate than that induced by the respective agent alone.
CONCLUSIONBortezomib can induce HL-60 cell apoptosis in a time- and dose-dependent manner, and a synergistic effect can be observed of bortezomib and arsenic trioxide in apoptosis induction.
