Preliminary study of neuroendocrine differentiation and its mechanism in ovarian epithelial tumors.
- Author:
Li-Yan JIANG
1
;
Zi-Neng WANG
;
Xin LUO
;
Jian-ping XU
;
Xing-mei XIE
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Aged; Case-Control Studies; Cell Differentiation; Female; Gene Expression Regulation, Neoplastic; Humans; Immunohistochemistry; Middle Aged; Neoplasms, Glandular and Epithelial; genetics; metabolism; pathology; Neuroendocrine Cells; metabolism; pathology; Ovarian Neoplasms; genetics; metabolism; pathology; Ovary; cytology; metabolism; pathology; Young Adult
- From: Journal of Southern Medical University 2007;27(7):1081-1083
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate neuroendocrine differentiation and its mechanism in ovarian epithelial tumors.
METHODSNeuroendocrine (NE) cells were identified by immunohistochemical staining for chromogranin A and synaptophysin in 79 cases of ovarian epithelial tumor and 22 cases of normal ovary. Double-labeling technique was used for simultaneous detection of CgA and epithelial membrane antigean (EMA), and the staining intensity was quantitatively evaluated using an image analysis system.
RESULTSThe positive staining rate for CgA and SYN in ovarian epithelial tumors was 59.4% and 65.36%, respectively, which was higher than that in normal ovary (P=0.000), in which numerous NE cells were found. Both the number and staining intensity of NE cells in ovarian epithelial tumor were increased as compared with normal ovary. Cells co-expressing CgA and EMA were detected in the ovarian epithelial tumors.
CONCLUSIONThe presence of NE cells in ovarian epithelial tumor suggests heterogeneity of the tumors, and the occurrence of "multidirectional differentiation cells" within the these tumors indicates that NE cells might derive from malignant cells with multidirectional differentiation capacity.
