Identification and tissue localization of intermediate filament protein in Angiostrongylus cantonensis.
- Author:
Jin-Xiu MENG
1
;
Ai HE
;
Mei CHENG
;
Gui-Feng XU
;
Zhou-Ya LI
;
Xi-Yong YU
;
Wen-Ling JIANG
;
Yun-Xiong LI
;
Xi-Mei ZHAN
Author Information
- Publication Type:Journal Article
- MeSH: Angiostrongylus cantonensis; cytology; metabolism; Animals; Cell Nucleus; metabolism; Electrophoresis, Polyacrylamide Gel; Intermediate Filament Proteins; classification; genetics; isolation & purification; metabolism; Protein Transport
- From: Journal of Southern Medical University 2007;27(8):1141-1144
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo identify the type of the intermediate filament (IF) protein of Angiostrongylus cantonensis and analyze its tissue localization.
METHODSRecombinant pET-IF of antigen IF was expressed in E.coli with IPTG induction, and the expression products were purified by His.Bind column and identified for determining the type of the IF protein by Western blotting. Anti-IF antibody was prepared by multi-spot subcutaneous injection into mouse and used to detect the tissue slices of A. cantonensis by immunohistochemical analysis.
RESULTSThe antigen IF were correctly expressed and purified, and identified as a keratin located in the intestine wall and cytoplusma.
CONCLUSIONThe antigen IF is distributed in the intestine wall of A. cantonensis.
