Cloning, screening and sequence analysis of the major allergens of Psilgramma menephorn.
- Author:
Yun LIU
1
;
Xiu-Zhen SUN
;
Wang-Gang ZHANG
;
Wei LI
;
Xiang-Li FENG
;
Jing XU
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Allergens; genetics; immunology; Animals; Cloning, Molecular; Electrophoresis, Polyacrylamide Gel; Female; Gene Library; Genes, Insect; Humans; Immunization; Lepidoptera; genetics; Male; Rabbits; Sequence Analysis, DNA
- From: Journal of Southern Medical University 2007;27(8):1145-1148
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo identify and isolate the genes encoding the allergens of Psilgramma menephorn by screening the cDNA expression library.
METHODSThe cDNA expression library of Psilgramma menephorn was constructed in lambdaZAPIIphage, and the library was screened using the sera from the patients allergic to Psilgramma menephorn and those from the rabbits immunized with Psilgramma menephorn extracts. The positive clones were subcloned into pBluescript plas, and the cDNA in the positive clones were amplified with PCR and sequenced.
RESULTS AND CONCLUSIONFive positive clones were obtained by immunological screening of 5 x 10(4) recombinants. Sequence analysis showed that the positive clones contained the new genes of Psilgramma menephorn allergens. This success in isolating these genes may facilitate the development of specific immunotherapy against Psilgramma menephorn allergy and further research of allergic diseases.
