Identification of differentially expressed genes in primary cultured nasopharyngeal carcinoma cells by cDNA microarray.
- Author:
Rui-Ping LI
1
;
Jian-Yong SHAO
;
Ling DENG
;
Mu-Sheng ZENG
;
Li-Bing SONG
;
Man-Zhi LI
;
Qiu-Liang WU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cells, Cultured; Gene Expression Profiling; Humans; Immunohistochemistry; Nasopharyngeal Neoplasms; genetics; pathology; Oligonucleotide Array Sequence Analysis; Polymerase Chain Reaction; RNA; isolation & purification
- From: Journal of Southern Medical University 2007;27(8):1156-1160
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo analyze the global gene expression profile of primary cultured nasopharyngeal carcinoma (NPC) cells using cDNA microarray techniques to screen new candidate genes related to the occurrence and progression of NPC.
METHODSA NPC cell line C666 and primary cultured NPC cells from biopsy specimens in 5 cases were analyzed with microarray techniques in comparison with 3 normal nasopharyngeal epithelial (NPE) biopsy specimens. Several differentially expressed genes identified from the microarray results were verified by fluorescence real-time PCR (FQ-PCR) and immunohistochemistry (IHC).
RESULTSPrimary cultured cells of both NPC and NPE were verified by cytokeratin IHC, EBER1 in situ hybridization and EBV-DNA real-time PCR. Compared with NPE cells, a total of 493 genes in at least 4/6 of the samples were identified to be differentially expressed in the primary cultured NPC cells, including 264 up-regulated and 229 down-regulated ones. Several differentially expressed genes according to the microarray results were confirmed by real-time PCR and IHC.
CONCLUSIONcDNA microarray technique provides an effective and accurate means for global gene expression profiling of primary cultured NPC cells to screen the differentially expressed genes, which may serve as an important basis for studying the mechanism, classification and diagnosis of NPC at the molecular level.
